Quantitative estimation of sitagliptin and dapagliflozin propanediol monohydrate in synthetic mixture using 1 order derivative spectroscopy simultaneous spectrophotometric analysis

Current research paper describes highly specific and reproducible 1 order derivative spectroscopic method for quantitative analysis of Sitagliptin which is a DPP4 inhibitors Dapagliflozin SGLT2 from its synthetic mixture. Both drugs are Anti Diabetics class. Present analytical was developed on Shimadzu double beam spectrophotometer equipped with UV probe 2.42 as software using methyl alcohol solvent. Quantification carried out at zero cross over point that 275 nm Dapagliflozin, it achieved 232 Sitagliptin. Method shows linear response in the range 25-125 µg/mL 2.5-12.5 Dapagliflozin. found to be accurate recovery between 99.3 – 100.1 % 98.2 100.7 The validated per ICH Q2 R1 guidelines successfully applied mixture